Number №2, 2019 - page 34-37

Development of laboratory technology for obtaining the cellular (erythrocyte) form of ceftriaxone and experimental substantiation of the feasibility of its use in non-obstructive pyelonephritis DOI: 10.29188/2222-8543-2019-11-2-34-37

Bratchikov O.I., Siplivyy G.V., Ipatov V.Yu., Bez'yazychnaya A.A., Kukureka A.V., Siplivaya L.E.
Information about authors:
  • Bratchikov O.I. -Dr. Sc., professor, Head of the Department of Urology KSMU of Ministry of Health of Russia, Kursk, Russia, bratov45@mail.ru
  • Siplivyj G.V. – Dr. Sc., professor at the Department of Urology KSMU of Ministry of Health of Russia, Kursk, Russia, SiplivyjGV@kursksmu.net
  • Ipatov V. Yu. – postgraduate student at the Department of Urology KSMU of Ministry of Health of Russia, Kursk, Russia
  • Bezieiazychnaya A.A. – postgraduate student at the Department of Pharmaceutical, Toxicological and Analytical Chemistry KSMU of Ministry of Health of Russia, Kursk, Russia
  • Kukureka A.V. – PhD, associate professor at the Department of Pharmaceutical, Toxicological and Analytical Chemistry KSMU of Ministry of Health of Russia, Kursk, Russia, KukurekaAV@kursksmu.net, ORCID 0000-0002-5244-0636
  • Siplivaya L.E. – Dr. Sc., professor, Head of the Department of Pharmaceutical, Toxicological and Analytical Chemistry KSMU of Ministry of Health of Russia, Kursk, Russia, farmchim@rambler.ru
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Introduction. Ceftriaxone is an effective cephalosporin antibiotic, but its use can lead to undesirable effects. Directed transport of drugsreducesthese effects, the therapeutic dose of the drug and the frequency of administration. Aim. To develop a ceftriaxone delivery system using erythrocyte vectors for non-obstructive pyelonephritis to selectively affect the organ in order to increase its efficiency and reduce toxicity

Materials and methods. Studies conducted on rats, non-obstructive pyelonephritiswas modeled by intraperitoneal administration of arachidonic acid. The state of the excretory function of the kidneys was judged by the concentration of urea and creatinine in the blood. To incorporate ceftriaxone into the erythrocyte stroma, the method of hypoosmotic hemolysiswas used. Ceftriaxone was quantitated by HPLC.

Results. It was established that five-fold administration of ceftriaxone to animals with pyelonephritis normalized body temperature for 10-12 days, excretory kidney function for 12-14 days.The number of leukocytes wasincreased, the number of lymphocytes decreased, the number of neutrophils did not change, decreased, but the imbalance of enzyme activity in the kidneys did not normalize.Asingle administration of erythrocyte form of ceftriaxone to ratswith pyelonephritis normalized body temperature for 5-7 days, excretory kidney function – for 5-8 days, respectively, on the 10th day full normalization of the number of leukocytes, lymphocytes, neutrophilsin the blood, activity was noted enzymes in the kidney.

Conclusion.Thus, a ceftriaxone delivery system was developed using erythrocyte carriers for non-obstructive pyelonephritis.

Authors declare lack of the possible conflicts of interests.

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