Expression of molecular markers of the acute kidney injury in the dynamics of experimental ischemia

O.I. Kit, E.M. Franciyanc, S.N. Dimitriadi, I.V. Kaplieva, L.K. Trepitaki

Nowadays the role of the organ-sparing kidney operations is growing, which is related to the prevention of the chronic kidney disease progression. The optimization of the diagnosis and prognosis of the development of acute kidney injury (AKI) is linked to the detection of certain biological markers. Among them are neutrophil gelatinaseassociated lipocalin-NGAL (lipocalin-2), interleukin-18, molecule of the renal damage-1 (3-NHE-3) and the enzymes localized normally in the brush border of the proximal channels, and some other parameters.

The aim of the study was to study the level of the AKI biomarkers and the functional parameters of the cortical cells in the kidneys of rats of different age and gender in the dynamics of acute ischemia. In the experiment 130 male and female inbreed rats were used with the body mass of 180-200 g (young animals) and 300-350 g (mature animals). The IFA analysis was done in the cortical tissue of the ischemic kidney with regard to expression of cystatin C, interleukin-18, lipocalin-2, L-FABP and KIM-1. It was shown, that in the cortical tissue of the ischemic kidneys in young males and old females early in the course (already 15 min after beginning) of ischemia accumulate cystatin C, interleukin-18, and L-FABP, in old males – this processes occur later (in 20 min). The alterations of the KIM-1 and lipocalin-2 levels could be observed even later. It is worth saying, that most sensitive to the ischemic damage were young male and old female rats, following by old males. The most tolerant were young females.

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