Number №4, 2019 - page 26-30

Positive experience of transplantation of a multicomponent composite containing allogeneic mesenchymal stem cells after resection of the rabbit bladder wall (description of experiment) DOI: 10.29188/2222-8543-2019-11-4-26-30

For citation: Orlova N.V., Muravyov A.N., Vinogradova T.I., Yudintseva N.M., Naschekina Yu.A., Zabolotnykh N.V., Lebedev A.A., Sheikhov M.G., Yablonsky P.K. Positive experience of transplantation of a multicomponent composite containing allogeneic mesenchymal stem cells after resection of the rabbit bladder wall (description of experiment). Experimental and clinical urology 2019;(4):26-30
Orlova N.V., Murav'ev A.N., Vinogradova T.I., Yudinceva N.M., Naschekina Yu.A., Zabolotnyh N.V., Lebedev A.A., Sheyhov M.G., Yablonskiy P.K.
Information about authors:
  • Orlova N.V. – Researcher, Department of Urology, Gynecology and Abdominal Surgery of FSBI SPb Research Institute of phthisiopulmonology of the Russian Ministry of Health, nadinbat@gmail.com; ORCID 0000-0002-6572-5956
  • Muravyev A.N. – PhD, Head of the Department of Urology, Gynecology and Abdominal Surgery of FSBI SPb Research Institute of phthisiopulmonology of the Russian Ministry of Health, urolog5@gmail.com, ORCID 0000-0002-6974-5305
  • Vinogradova T.I. – Dr. Sc., professor, head of the laboratory of experimental tuberculosis and new medical technologies of FSBI SPb Research Institute of phthisiopulmonology of the Russian Ministry of Health, vinogradova@spbniif.ru, ORCID 0000-0002-5234-349X
  • Yudintseva N.M. – PhD, Senior Researcher of FSBI of Cytology of RAS, yudintceva@mail.ru, ORCID 0000-0002-7357-1571
  • Naschekina Yu.A. – PhD, Researche of FSBI of Cytology of RAS, ulychka@mail.ru
  • Zabolotnykh N.V. – Dr. Sc., Leading Researcher of the laboratory of experimental tuberculosis and new medical technologies of FSBI SPb Research Institute of phthisiopulmonology of the Russian Ministry of Health
  • Lebedev A.A. – PhD, senior researcher of Department of Urology, Gynecology and Abdominal Surgery of FSBI SPb Research Institute of phthisiopulmonology of the Russian Ministry of Health, dialog10.65@mail.ru
  • Sheikhov M.G. – graduate student of FSBI SPb Research Institute of phthisiopulmonology of the Russian Ministry of Health, sheykhov@mail.ru
  • Yablonsky P.K. – Dr. Sc., professor, director of FSBI SPb Research Institute of phthisiopulmonology of the Russian Ministry of Health, Dean of the Faculty of Medicine, St. Petersburg State University, glhirurgb2@mail.ru
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Introduction: The evaluation of the possible use a multicomponent graft with allogeneic cells to replace a defect in the bladder wall under experimental conditions.

Materials and methods: The rabbit bone marrow mesenchymal stromal cells were isolated and cultured according to the standard method. A multicomponent composite based on a polylactide matrix was colonized by allogeneic cells and transplanted in vivo to a model of a partial resection of a rabbit bladder.

Results: Full bladder with normal capacity is visible on a series of MRI images after 2.5 months. The guiding artifact from the iron-containing labels introduced into the cells is visualized at the implantation site. Labeled cells taking part in the formation of a structure similar to urothelium are identified at the site of implantation using confocal microscopy of cryosections.

Conclusions: The experiment shows again the need for further research in the field of bladder wall reconstruction. The development of methods for creating a multicomponent graft using allogeneic cells can help improve the treatment results for pathologies where impossible to obtain autologous material. The recent results, although encouraging, require more detailed study.

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allogeneic transplantation, bone marrow mesenchymal stromal cells, bladder, tissue engineering

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