Two-sided nephroprotective effect of novokain blockade in the experiment with one-sided ischemia-reperfusion of the kidney

Kit O.I., Franciyanc E.M., Dimitriadi S.N., Kaplieva I.V., Trepitaki L.K., Shvyrev D.A., Cheryarina N.D., Pogorelova Yu.A.

Acute renal damage is a frequent consequence of the prolonged warm kidney ischemia during the operation, which could affect the contralateral kidney being estimated as healthy before the operation. 

The aim of this study was the investigation of the one-side ischemia-reperfusion effect on the both kidneys and of the nephroprotective action of the novokain blockade in these conditions. The study model was “two kidney – one clamp”. The splanchnic plexus blockade using 0.1 ml of 2% novokain solution was carried out through the injection on the left side paranephric. Sixty white inbreed female and male mice were used (body weight 200-250 g for young and 300-350 g for old animals, respectively). Interleukin 18 (IL-18) and L type protein binding the fatty acids (L-FABP) concentrations were measured using ELISA. Monolateral experimental ischemia (20 minutes) led to the changes of the markers’ concentration in both kidneys. Intensity and dynamics of the alterations were dependent on the sex and age of the animals. Novokain blockade of the splanchnic branches showed protective effect on the distal renal channels of both kidneys, stemming from ischemia-reperfusion damage. 

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interleukin 18, L-FABP,enzyme-linked immunosorbent assay (ELISA),experimentalwarmischemia,contralateral kidney,splanchnic plexus blockade