18+

 

Number №2, 2021 - page 14-22

Оксидантный стресс и тканевая гипоксия как факторы, способствующие развитию дисфункции предстательной железы и мочевого пузыря при метаболическом синдроме DOI: 10.29188/2222-8543-2021-14-2-14-22

For citation: Kirpatovsky V.I., Golovanov S.A., Drozhzheva V.V., Kudryavtseva L.V., Frolova E.V., Vekilyan M.A. Oxidative stress and tissue hypoxia as factors contributing to the development of prostate and bladder dysfunction in metabolic syndrome. Experimental and Clinical Urology, 2021;14(2):14-22; https://doi.org/10.29188/2222-8543-2021-14-2-14-22
Kirpatovskiy V.I., Golovanov S.A., Drozhzheva V.V., Kudryavceva L.V., Frolova E.V., Vekil'yan M.A., Kaprin A.D.
Information about authors:
  • Kirpatovsky V.I. – Dr. Sc., professor, сhief scientific Researcher of N. Lopatkin Research Institute of urology and Interventional Radiology – branch of the National Medical Research Centre of Radiology of Ministry of health of Russian Federation; Moscow, Russia; vladkirp@yandex.ru; https://orcid.org/0000-0002-4356-9200
  • Golovanov S.A. – Dr. Sc., head laboratory diagnostic group of Scientific Laboratory Department of N.A. Lopatkin Research Institute of Urology and Interventional Radiology – Branch of the National Medical Research Radiological Centre of the Ministry of Health of Russian Federation; Moscow, Russia; sergeygol124@mail.ru; https://orcid.org/0000-0002-6516-4730
  • Drozhzheva V.V. – researcher of scientific Laboratory Department of N.A. Lopatkin Research Institute of Urology and Interventional Radiology – Branch of the National Medical Research Radiological Centre of the Ministry of Health of Russian Federation; Moscow, Russia
  • Kudryavtseva L.V. – Senior Lecturer of Pathological Anatomy Department of Medical Institute of Russian Peoples' Friendship University; Moscow, Russia; kgosha@mail.ru
  • Frolova E.V. – Chief researcher, Department of «Biology» of All-Russian Institute of Scientific and Technical Information of RAS; Moscow, Russia; vladkirp@yandex.ru
  • Vekilyan M.A. – PhD, chief physician of N. Lopatkin Research Institute of urology and Interventional Radiology – branch of the National Medical Research Centre of Radiology of Ministry of health of Russian Federation; Moscow, Russia
248

Introduction. The development of oxidative stress and nonspecific inflammation is one of the leading factors in the development of benign prostatic hyperplasia (BPH) and associated urination disorders in metabolic syndrome (MS). However, the specific mechanisms of these processes are not entirely clear.

The purpose of the study. To study the activity of reactive oxygen species production and the functional state of mitochondria in the prostate and bladder and their role in the dysfunction of these organs using an experimental model of MS induction in rats.

Material and methods. In 10 adult mongrel male rats MS was induced by keeping them on a high-calorie diet with an increased content of carbohydrates and fats for 3 months. 10 rats kept on a standard vivarium diet served as controls. The development of MS was confirmed by characteristic changes in the biochemical analysis of blood (hyperglycemia, hyperuricemia, dyslipidemia, hyperinsulinemia). In both series of rats, sections of the native prostate and bladder were examined by laser confocal microscopy and stained with fluorescent probes that characterize the activity of the production of reactive oxygen species (dichlorofluorescein-DCF) and the functional state of the mitochondria (tetramethylrodamine ether – TMRE). The activity of a number of intracellular enzymes (AST, ALT. Alkaline phosphatase, LDH) was investigated in the tissues and urine.

Results. In rats with MS, the development of BPH and hypertrophy of the bladder were revealed, confirmed histologically. The study of sections of both organs by confocal microscopy revealed a significant increase in the production of reactive oxygen species by their cells and a decrease in the functional activity of mitochondria, which indicated the development of oxidant stress and tissue hypoxia. In the prostate, this was accompanied by a decrease in the secretory activity of the prostate glands, and in the bladder – the release of cytoplasmic enzymes from damaged cells into the urine, indicating cell damage.

Conclusion. The causes of the development of a non-specific inflammatory process in the prostate and bladder, leading to dysfunction of these organs in MS, are increased production of reactive oxygen species and the development of tissue hypoxia

Conflict of interest. The authors declare no conflict of interest.

AttachmentSize
Скачать статью3.46 MB
metabolic syndrome; oxidant stress; confocal microscopy; prostate; benign prostatic hyperplasia; urinary bladder

Readera - Социальная платформа публикаций

Crossref makes research outputs easy to find, cite, link, and assess