DOI: 10.29188/2222-8543-2024-17-2-36-44
For citation:
Faniev M.V., Kadyrov Z.A., Druzhinina N.K., Stepanov V.S., Prokopyev Y.V., Fedorenko T.V., Markelova M.I., Khusnutdinova D.R.,
Grigorieva T.V. The structure of the microbiota of fertile and infertile patients with different types of azoospermiа. Experimental and Clinical Urology 2024;17(2):36-44; https://doi.org/10.29188/2222-8543-2024-17-2-36-44
Faniev M.V.
Kadyrov Z.A.
Druzhinina N.K.
Stepanov V.S.
Prokop'ev Ya.V.
Fedorenko T.V.
Markelova M.I.
Husnutdinova D.R.
Grigor'eva T.V.
Information about authors:
- Faniev M.V. – PhD, Associate Professor, Department of Endoscopic Urology, RUDN University; Moscow, Russia; RSCI Author ID 1056145, https://orcid.org/0000-0002-7323-3126
- Kadyrov Z.A. – Dr. Sci., Professor, Head of the Department of Endoscopic Urology at RUDN University; Moscow, Russia; RSCI AuthorID 721133, https://orcid.org/0000-0002-1108-8138
- Druzhinina N.K. – postgraduate student of the Department of Endoscopic Urology and Ultrasound Diagnostics of the Faculty of Continuing Medical Education of the Medical Institute of the RUDN University; Moscow; Russia; RSCI Author ID 1074829, https://orcid.org/0000-0003-3277-6068
- Stepanov V.S. – leading specialist urologist of the medical holding SM Clinic; Moscow, Russia; https://orcid.org/0000-0003-0525-3026
- Prokopyev Ya.V. – PhD, Associate Professor of the Department of Urology and Nephrology of the Kazan State Medical Academy – branch of the Russian Medical Academy of Continuing Professional Education of the Ministry of Health of Russia; Kazan, Russia; https://orcid.org/0000-0002-4345-127X
- Fedorenko T.V. – PhD, biologist of the Laboratory of Cell Technologies of the Center for Reproductive and Cellular Medicine of the Diagnostic Center of the State Budgetary Healthcare Institution «DGKB in Krasnodar» of the Ministry of Health of the Republic of Kazakhstan; Krasnodar, Russia; RSCI Author ID 706354, https://orcid.org/0009-0008-9780-4158
- Markelova M.I. – junior researcher at OpenLab «Omics Technologies» of the Institute of Fundamental Medicine and Biology of the Federal State Autonomous Educational Institution of Higher Education «Kazan (Volga Region) Federal University»; Kazan, Russia; RSCI Author ID 976359, https://orcid.org/0000-0001-7445-2091
- Khusnutdinova D.R. – Chief Project Engineer of the Interdisciplinary Center for Proteomic Research, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University; Kazan, Russia; RSCI Author ID 877569, https://orcid.org/0000-0002-9982-9059
- Grigorieva T.V. – PhD, senior researcher at OpenLab «Omics Technologies» of the Institute of Fundamental Medicine and Biology of the Federal State Autonomous Educational Institution of Higher Education «Kazan (Volga Region) Federal University»; Kazan, Russia; Author ID 616577, https://orcid.org/0000-0001-5314-7012
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Introduction. The study of the human microbiome has become of increasing scientific interest. A balanced and diverse microbiota of the male re- productive system is essential for optimal reproductive health. Today, there are no diagnostic tests to determine the effect of specific microorganisms on the quality of seminal fluid, however, the use of next-generation sequencing (NGS), using the 16S rRNA gene sequence as a molecular marker, provides us with the opportunity to understand the complexity of the relationship between the microbiome and its host.
The aim of the study. To conduct a comparative analysis of the taxonomic structure of the microbiota between patients with obstructive and nonobstructive azoospermia and the fertility group (patients with a history of having children).
Materials and methods. The study included (n=57) patients (infertile patients with obstructive azoospermia (OA), non-obstructive azoospermia (NOA) and a control group (FERT). All patients underwent percutaneous aspiration of sperm from the testicle (testicular sperm aspiration, MESA), with subsequent in vitro fertilization using ICSI technology, or cryopreservation of biological material. The amplicons of the bacterial 16S rRNA gene were analyzed using high-performance next-generation sequencing (NGS). For this research of bacterial composition of the urogenital tract and control the purity of the method, samples has been taken from the urethra of each patient who underwent testicular biopsy. The data was processed using the QUICKTIME program (version 1.9.1).
Results. No significant differences were found in the comparison groups during the study of the urethral bacterial composition. The species diversity, as well as the total number of detected taxonomic units (OTE) in the NOA and OA groups are significantly lower than in the FERT group. The presentation of the phylum like Proteobacteria, Firmicutes, Bacteroidetes, Bifidobacterium adolescentis in the studied groups is different. Three microorganisms were identified with the same frequency in the urethra and testicular tissue (the exact Fisher criterion was used, adjusted for multiple comparison by the Benjamin-Hochberg method). The representation of the phylum Prevotella, Comamonadaceae, Veillonella dispa, Comamonadaceae in the studied groups differs.
Conclusion. Testicular tissue is not sterile and has its own unique microbial landscape for each comparison group. Bacterial communities of testicular tissue in patients with azoospermia are characterized by a reduced diversity and a specific composition that differs from the urethral microbiota. These results may be useful in further to study the role of microbiota in the pathology of spermatogenesis and developing new approaches to the treatment and diagnosis of male infertility.
