Sholan Rashad Farhad oglu – Doctor of Medical Sciences; Head of the Department of Kidney Diseases and Transplantology, Republican Medical and Diagnostic Center of the Ministry of Health of the Republic of Azerbaijan, Baku, Azerbaijan Republic, firstname.lastname@example.org ORCID 0000-0002-1047-167X
Contacts: Sholan Rashad Farchad ogly, email@example.com
Aim – to evaluate levels of nerve growth factor (NGF) in blood and urine in different experimental models of interstitial cystitis / bladder pain syndrome (IC / BPS).
Material and methods. IC / BPS was modeled on white New Zealand female rabbits with 1500-2000 g body mass using different techniques: group I (n=8) – injection of 70% alcohol solution into bladder cavity; group II (n=7) - injection of protamine sulfate into bladder cavity; group III (n=8) - injection of 0,5% solution of hydrochloric acid (HCl) into urinary bladder; group IV (n=15) - injection of urine taken from an animal’s bladder into bladder wall; group V (n=7) - injection of 0,9% sodium chloride (NaCl) solution into bladder wall; group VI (n=8) – intact rabbits (control group). Nerve growth factor was measured in blood and urine of animals using enzyme immunoassay (ELISA) 1 day and 14 days after the beginning of experiment.
Results. At the 1st day of experiment blood level of NGF in all groups was statistically significantly higher than in control group, while only in group IV its level was elevated in urine (by 68,2%, р<0,01). At 14 days biomarker elevation in blood persisted in all groups when compared with NGF level in intact animals (р<0,05). At 14 days in group IV a significant rise in NGF level was observed in blood and urine when compared with baseline, by 65,5% (p<0,01) and 52,7% (p<0,05), respectively. Strong positive statistically significant correlation between NGF concentrations in blood and urine was observed in group II at day 14.
Conclusion. Rabbit models of IC/BPS in all groups had statistically significant elevation of nerve growth factor concentration in blood. Among the commonly used experimental models of IC/BPS the most stable one is created by injecting urine into bladder wall.
For citation: Sholan R.F. Comparative evaluation of the level of nerve growth factor in experimental models of interstitial cystitis / bladder pain syndrome. Experimental and clinical urology 2019;(3):178-181