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Number №3, 2021 - page 18-27

Experimental evaluation of the influence of isobornylphenols on the development of BPH and the redox potential of prostate cells DOI: 10.29188/2222-8543-2021-14-3-18-26

For citation: Borovskaya T.G., Kamalova S.I., Kuchin A.V., Chukicheva I.Yu., Buravlev E.V., Krivova N.A., Zaeva O.B., Vychuzhanina A.V., Shchemerova Yu.A., Grigorieva V.A., Neplokhov E.A.,Vasilevsky R.P., Poluektova M.E. Experimental evaluation of the influence of isobornylphenols on the development of BPH and the redox potential of prostate cells. Experimental and Clinical Urology, 2021;14(3):18-26; https://doi.org/10.29188/2222-8543-2021-14-3-18-26
Borovskaya T.G., Kamalova S.I., Kuchin A.V., Chukicheva I.Yu., Buravlev E.V., Krivova N.A., Zaeva O.B., Vychuzhanina A.V., Schemerova Yu.A., Grigor'eva V.A., Neplohov E.A., Vasilevskiy R.P., Poluektova M.E.
Information about authors:
  • Borovskaya T.G. – Dr. Sc., Professor, Head of the Laboratory of pharmacology of the reproductive system, Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of the Russian Academy of Sciences; Tomsk, Russia; https://orcid.org/0000-0002-0651-4841
  • Kamalova S.I. – PhD. of Biological Sciences, Research Assistant of laboratory of pharmacology of the reproductive system, Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of the Russian Academy of Sciences; Tomsk, Russia; https://orcid.org/0000-0001-6403-7193
  • Kuchin A.V. – Dr. Sc (Chem.), Project Leader, Institute of Chemistry, Komi Scientific Centre, Ural Branch of the Russian Academy of Sciences; Syktyvkar, Russia; https://orcid.org/0000-0003-4322-7961
  • Chukicheva I.Yu. – DSc (Chem.), Project Leader, Institute of Chemistry, Komi Scientific Centre, Ural Branch of the Russian Academy of Sciences; Syktyvkar, Russia; https://orcid.org/0000-0002-7346-0812
  • Buravlev E.V. – PhD (Chem.), Senior Researcher, Institute of Chemistry, Komi Scientific Centre, Ural Branch of the Russian Academy of Sciences; Syktyvkar, Russia; https://orcid.org/0000-0002-7580-982X
  • Krivova N. A. – Dr. Sc., Professor, Head. laboratory of experimental physiology of a separate structural unit "Research Institute of Biophysics Biology, Tomsk State University"; Tomsk, Russia; https://orcid.org/0000-0002-3965-8665
  • Zaeva O. B. – PhD, researcher laboratory of Experimental Physiology of a separate structural unit «Research Institute of Biophysics Biology, Tomsk State University»; Tomsk, Russia
  • Vychuzhanina А.V. – Ph.D, Senior Research Officer of laboratory of pharmacology of the reproductive system, Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of the Russian Academy of Sciences; Tomsk, Russia; https://orcid.org/0000-0001-6151-0985
  • Shchemerovа Y.A. – Ph.D. of Biological Sciences, Research Officer of laboratory of pharmacology of the reproductive system, Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of the Russian Academy of Sciences; Tomsk, Russia; https://orcid.org/0000-0002-0895-9000
  • Grigorieva V.A. – Research Assistant of laboratory of pharmacology of the reproductive system, Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of the Russian Academy of Sciences; Tomsk, Russia; https://orcid.org/0000-0003-1863-088X
  • Neplokhov E.A. – MD, PhD, Regional State Autonomous Healthcare Institution «Regional Perinatal Center named after I.D. Evtushenko»; Tomsk, Russia
  • Vasilevsky R.P. – MD, Chief Freelance Specialist in Urology, Department of Healthcare in the South-West Administrative District, GBUZ City
  • Polyclinic No. 134 DZM; Moscow, Russia Poluektova M.E. – Ph.D. of Biological Sciences, Senior Research Officer of laboratory of pharmacology of the reproductive system, Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of the Russian Academy of Sciences; Tomsk, Russia; https://orcid.org/0000-0002-9052-3096
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Introduction. Benign prostatic hyperplasia (BPH) is a common urological disorder in older men. It is characterized by the development of glandularstromal hyperplasia of the prostate with the formation of new glandular structures and subsequent symptoms from the lower urinary tract. It has now been established that the pathogenesis of this disease is multifactorial and one of the possible mechanisms for the development of BPH is oxidative stress.

Purpose. Study of the effect of phenols with a bulky isobornyl substituent (2,6-diisobornyl-4-methylphenol and 4-hydroxymethyl-2,6-diisobornylphenol) on the growth of experimental BPH and the antioxidant balance of prostate cells in comparison with Prostamol Uno.

Materials and мethods. Experiments were carried out on 50 male Wistar rats. BPH was caused by daily administration of sulpiride (60 days) to male rats of late reproductive age. After 2 months, the animals were weighed and sacrificed in a CO2 chamber. The mass, mass coefficient, volume of the lateral lobe of the pancreas were determined, morphological analysis was performed. Investigated prooxidant and antioxidant activity. The results were processed by the method of variation statistics using the Mann-Whitney nonparametric U test.

Results. The efficacy of the investigated drugs in BPH decreased in the following sequence: sulpiride + substance 4-hydroxymethyl-2,6-diisobornylphenol (HDB) → sulpiride + substance Dibornol (DB) → sulpiride + Prostamol Uno (PU). When comparing the results of evaluating the anti-prooxidant status with the therapeutic effect of the studied drugs, it was found that isobornylphenols, which are highly effective as prostatotropic drugs, did not show a more significant effect, compared to PU, on the redox potential of prostatic tissue cells.

Conclusions. Drugs DB, HDB, PU have a normalizing effect on the level of severity of redox reactions in the sulpiride model of BPH.

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benign prostatic hyperplasia; isobornylphenols; antioxidant and prooxidant activity; rats; therapeutic activity; еxperimental model

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