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High-throughput sequencing of the testicular microbiome in patients with non-obstructive azoospermia and varicocele DOI: 10.29188/2222-8543-2023-16-2-38-43
For citation:
Faniev M.V., Prokopyev Ya.V., Faustova K.V., Kadyrov Z.A., Vodolazhsky D.I., Markelova M.I., Khusnutdinova D.R., Grigoryeva T.V.,
Karslyan E.M., Bersanova M.Kh., Akobova R. High-throughput sequencing of the testicular microbiome in patients with non-obstructive azoospermia and varicocele. Experimental and Clinical Urology 2023;16(2):38-43; https://doi.org/10.29188/2222‑8543‑2023‑16‑2‑38‑43
Faniev M.V. – Dr. Sci., Associate Professor of the Department of Endoscopic Urology, Federal State Autonomous Educational Institution of «RUDN University»; Moscow, Russia; RSCI Author ID 1056145; https://orcid.org/0000‑0002‑7323‑3126
Prokopiev Ya.V. – PhD, Associate Professor, Department of Urology and Nephrology, Kazan State Medical Academy; RSCI Author ID 1066425; Kazan, Russia
Faustova K.V. – Resident Physician, Department of Obstetrics and Gynecology, Kazan State Medical Academy; Kazan, Russia; https://orcid.org/0000‑0001‑8702‑0159
Kadyrov Z.A. – Dr. Sci., Professor, Head of the Department of Endoscopic Urology Federal State Autonomous Educational Institution of Higher Education «RUDN University»; RSCI Author ID 721133; Moscow, Russia
Vodolazhsky D.I. – PhD, Head of the Laboratory of Cell Technologies, Central Research Laboratory of the S.I. Georgievsky Medic Academy at V.I. Vernadsky Crimean Federal University; Simferopol, Russia; RSCI Author ID 289513; https://orcid.org/0000‑0003‑1114‑8732
Markelova M.I. – Researcher, Scientific Research Laboratory «Multimix Technologies of Liv- ing Systems», Kazan Federal University, Institute of Fundamental Medicine and Biology; Kazan, Russia; RSCI Author ID 976359; https://orcid.org/0000‑0001‑7445‑2091
Khusnutdinova D.R. – Researcher, Scientific Research Laboratory «Multimix Technologies of Living Systems», Kazan Federal University, Institute of Fundamental Medicine and Biology; Kazan, Russia; RSCI Author ID 877569; https://orcid.org/0000‑0002‑9982‑9059
Grigoryeva T.V. – PhD, Leading Researcher, Scientific Research Laboratory «Multimix Technologies of Living Systems», Kazan Federal University, Institute of Fundamental Medicine and Biology; Kazan, Russia; https://orcid.org/0000‑0001‑5314‑7012
Karslyan E.M. – member of the Department of Endoscopic Urology, Federal State Autonomous Educational Institution of Higher Education «RUDN University»; Moscow, Russia; https://orcid.org/0009‑0001‑7889‑4484
Bersanova M.Kh. – student, A.I. Yevdokimov Moscow State University of Medicine and Dentistry; Moscow, Russia
Akobova R. – student Federal State Autonomous Educational Institution of Higher Education «RUDN University»; Moscow, Russia
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Introduction. The male factor in infertile marriages accounts for about 50% of all cases, which is associated with changes in the characteristics of the ejaculate microbiota and directly correlates with changes in sperm parameters, including sperm count, motility, and morphology. Purpose of the work: to determine the presence of a correlation between the taxonomic diversity of the testicular microbiome and the urethral microbiome and its role in the development of infertility in patients with non-obstructive azoospermia against the background of existing varicose veins of the spermatic cord, using the new generation high-throughput sequencing (NGS) method.
Materials and methods. From 2020 to 2022, we studied and analyzed the biological material of 11 infertile men with non-obstructive azoospermia and concomitant varicocele. The resulting material of 11 urethral swabs and 10 testicular tissue samples was prepared and taken for further study of the bacterial microbiome using the high-throughput NGS method.
Results. Analysis of the studied clinical group clearly demonstrated that the testicular tissue of patients with azoospermia and concomitant varicocele has its own unique microbiome. This may indicate not only taxonomic diversity at the level of both phyla and reads, but also a high degree of significance of impaired function of the blood-testicular barrier (HTB).
Сonclusions. The testicular tissue of infertile men with azoospermia and concomitant varicocele has a pronounced diversity of microbiota not only at the level of phyla, but also at the level of reads, which proves the importance of the testicular microbiota in the regulation of spermatogenesis, and also confirms the theory of the initial insufficiency of the hematotesticular barrier against the background of the existing varicose veins of the seminal cord.
5-year impact factor of Russian science citation index from 15.01.2020 - 0,723
10-year H-index 21
Included in the Russian Science Citation Index (RSCI)
ISSN print 2222-8543 ISSN online 2712-8571
The journal is included in the List of Russian peer-reviewed scientific journals which should publish the main scientific results of dissertations for the degrees of Doctor and PhD of sciences
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