Influence of the experimental metabolic syndrome on the function of the urinary bladder in rats

Kirpatovskiy V.I., Mudraya I.S., Grekov E.A., Kabanova I.V., Golovanov S.A., Drozhzheva V.V., Nadtochiy O.N.

The experiments were made using 20 mature male rats (line “August”) with a mass of 280-320 g. In 10 rats metabolic changes typical for metabolic syndrome (MS) were induced using high-calorie diet (adding of 20% margarine to standard diet and 20% of fructose to the water during 3 months), bilateral orchiectomy (to create androgen deficiency) and stress (immobilization stress during 1 day in the end of the observation period). Another 10 rats were controls. In the experimental group increase of the body mass, hyperglycaemia, hyperglucosuria, hypertriglyceridemia (with the increase of low density lipoproteins), hyperuricemia and hyperuricuria were observed. These were typical for MS. In the rats with MS it was shown that spontaneous bladder contractions were enforced, with periodical increases in the intravesical pressure up to 7-12 cm H2O, while in the control group these contractions didn’t get over the point of 4 cm H2O. Also in vitro estimation of bladder fragments’ contraction showed that the amplitude of spontaneous contractions in resting conditions was higher in the group of rats with MS compared to control group. During the electrostimulation spontaneous contractions in the muscle fragments of the rats with MS were also registered between those electrically induced. This was not a case in the control group. Autonomic regulation using harmonic analysis of bioimpedance variation, which showed the dominancy of regional parasympathetic activity in the rats with MS.

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