Predictive value of DNA fragmentation index in sperm cells for the success of assisted reproductive techniques. Empirical antioxidant therapy for the correction of DNA fragmentation in the setting of pathological oxidative stress of the ejaculate 

Korshunov M.N., Korshunova E.S., Darenkov S.P.

Introduction: The level of sperm DNA integrity is a useful predictor of blastocyst quality and pregnancy rate. The high level of oxidative stress reaction (ROS) may cause sperm DNA damage. Antioxidant therapy might improve sperm quality and ART outcomes. The literature evidence of efficacy of antioxidant therapy in the correction of sperm DNA damage and high level of semen ROS in preparation before ART cycles after failure cases is limited. It was the aim of our study.

Material & Methods: The couples with male factor of infertility were investigated. 32 failures ART cases with a poor blastocyst formation rate and spontaneous abortions were selected.

All of the couples were planning subsequent IVF-ICSI cycles. Semen analysis, sperm DFI, ROS were performed before and after antioxidant therapy. After the therapy the couples underwent subsequent IVF-ICSI cycles.

Results: All the patients had severe pathospermia, abnormal DFI and ROS before the treatment. The quality of sperm, level of DNA fragmentation and ROS were improved after the therapy. Biochemical and clinical pregnancy rates were 65,6% and 53,1% respectively. In 15 cases live birth rate were fixed (46,8%).

Conclusions: This study shows that evaluation of sperm DNA fragmentation is an important method in prognosis of ART success. Antioxidant therapy is an effective and safety method in the correction of idiopathic sperm DNA damage and pathological level of sperm ROS. That may improve embryo quality and pregnancy rate.

Authors declare lack of the possible conflicts of interes

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sperm DNA fragmentation, oxidative stress reaction, assisted reproductive technique, antioxidants