Number №3, 2019 - page 26-31

Extracellular DNA in benign prostatic hyperplasia in old and young rats DOI: 10.29188/2222-8543-2019-11-3-26-31

Vasil'eva I.N., Bespalov V.G., Semenov A.L., Tochil'nikov G.V., Romanov V.A., Aleksandrov V.A.
Information about authors:
  • Vasilieva I.N. – Ph.D., Senior Researcher, Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N. Petrov National Medical Research Center of Oncology of the Ministry of Health of Russia; iravasilyeva@hotmail.com; ORCID 0000-0003-1440-9934
  • Bespalov V.G. – Dr.Sc., Head of the Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N. Petrov National Medical Research Center of Oncology of the Ministry of Health of Russia, Professor of the International Scientific Center "Biotechnology of the third Millennium" of the ITMO University;
  • bespalov_niio@mail.ru; ORCID 0000-0003-4976-8515
  • Semenov A.L. – Researcher, Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N. Petrov National Medical Research Center of Oncology of the Ministry of Health of Russia; genesem7@gmail.com; ORCID 0000-0002-5190-0629
  • Tochilnikov G.V. – Ph.D., Researcher, Laboratory of Chemoprevention and Oncopharmacology, N.N. Petrov National Medical Research Center of Oncology of the Ministry of Health of Russia; gr75@mail.ru
  • Romanov V.A. Laboratory Assistant, Laboratory of Chemoprevention and Oncopharmacology, N.N. Petrov National Medical Research Center of Oncology of the Ministry of Health of Russia; vovavarxan@yandex.ru
  • Alexandrov V.A. -– Dr.Sc., Professor of the Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N. Petrov National Medical Research Center of Oncology of the Ministry of Health of Russia; alexandrov.valeri@yandex.ru
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Contacts: Vasilieva Irina Nikolaevna, iravasilyeva@hotmail.com Introduction. Extracellular DNA (ecDNA) is considered to be a biomarker of cell death in diagnostics of prostate diseases. Aim – To assess concentration of ecDNA in blood of young and elderly rats in normal conditions and in induced benign prostatic hyperplasia (BPH).

Materials and methods. Male Wistar rats were divided into 4 groups – young (3 months) and elderly (20 months), intact and with induced BPH. Rats in BPH groups were surgically castrated and administered testosterone (25 mg/kg, 7 injections during 20 days). When the experiment was over, testosterone and ecDNA levels in blood, body weight and prostate weight, prostatic index (PI) and histological features were evaluated.

Results. Among intact rats the elderly had significantly higher level of ecDNA (2,00±0,14 ng/mcl) compared to the young (1,02±0,30 ng/mcl). Body weight of elderly rats was 1,6 times higher, prostate weight was 1,4 times higher than in young rats, without increase in PI. Blood testosterone level in young rats (6,20±0,93) was 1,6 times higher compared to old rats (3,77±0,55). Elderly rats had focal prostatic hyperplasia. Among BPH rats the elderly similarly had significantly higher level of ecDNA (3,14±0,76 ng/mcl) compared to the young (0,80±0,14 нг/мкл). Body weight in old rats was 1,8 times higher, prostate weight was 2,3 times higher than in young rats, and PI was increased. Blood testosterone level in young (15,76±0,51) and elderly (16,99±1,10) rats didn’t differ significantly. Young and old rats had morphological signs of BPH. When BPH was induced, judging by the ecDNA level, processes of cell death didn’t change in young animals, but were significantly amplified in old animals. Similar trend, though less pronounced, was observed among intact rats. Conclusion. The data is an evidence of amplified apoptosis in BPH when tissue grows within prostate itself.

Key words: benign prostatic hyperplasia, testosterone, apoptosis, extracellular DNA.

For citation: Vasilievа I.N., Bespalov V.G., Semenov A.L., Tochilnikov G.V., Romanov V.A., Alexandrov V.A. Extracellular DNA in benign prostatic hyperplasia in old and young rats. Experimental and clinical urology 2019; (3):26-31

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